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OPTIMIZATION OF PSEUDOMONAS PUTIDA SOL10 BIOSURFACTANT PRODUCTION USING COSTEFFECTIVE SUBSTRATES

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01 Pages : 1-14

http://dx.doi.org/10.31703/gdddr.2023(VIII-IV).01      10.31703/gdddr.2023(VIII-IV).01      Published : Dec 2023

Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates

Abstract

    Natural resources as a substitute energy source in recent technological advancements have resulted in the production of biosurfactants. In order to reduce production costs and maximise yield, the current work replaces expensive yeast extract with less expensive substrates and optimises temperature, pH, and agitation. Pseudomonas putida SOL10 produced biosurfactants using a variety of substrates, such as red beans, molasses, whey, potato peels, olive oil, castor oil, crude oil, kerosene oil, and leftover cooking oil. These sources were utilised singly with an inducer, urea. Surface tension and oil displacement assays were used to measure the generation of biosurfactants. The combination of 1% potato peels, 2% leftover cooking oil, and 0.1% urea produced the highest amount of biosurfactant at 30ËšC, 9.5 pH, and 150 rpm of agitation. There was a drop in surface tension of up to 29 mN/m with a 6.7 cm ODA zone.

    Biosurfactants, Biodegradability, Yeast Extract, Pseudomonas Putida SOL10

    (1) Bushra Rehman
    Assistant Professor, Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda, KP, Pakistan.
    (2) Sadia Aslam
    Assistant Professor, Department of Microbiology, Quaid I Azam University Islamabad, Pakistan.
    (3) Safia Ahmed
    Department of Microbiology, Quaid I Azam University, Islamabad, Pakistan.
    (4) Hira Ikram
    Lecturer, Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda, KP, Pakistan.
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Cite this article

APA

    APA : Rehman, B., Aslam, S., & Ahmed, S. (2023). Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates. Global Drug Design & Development Review, VIII(IV), 1-14. https://doi.org/10.31703/gdddr.2023(VIII-IV).01

CHICAGO

    CHICAGO : Rehman, Bushra, Sadia Aslam, and Safia Ahmed. 2023. "Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates." Global Drug Design & Development Review, VIII (IV): 1-14 doi: 10.31703/gdddr.2023(VIII-IV).01

HARVARD

    HARVARD : REHMAN, B., ASLAM, S. & AHMED, S. 2023. Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates. Global Drug Design & Development Review, VIII, 1-14.

MHRA

    MHRA : Rehman, Bushra, Sadia Aslam, and Safia Ahmed. 2023. "Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates." Global Drug Design & Development Review, VIII: 1-14

MLA

    MLA : Rehman, Bushra, Sadia Aslam, and Safia Ahmed. "Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates." Global Drug Design & Development Review, VIII.IV (2023): 1-14 Print.

OXFORD

    OXFORD : Rehman, Bushra, Aslam, Sadia, and Ahmed, Safia (2023), "Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates", Global Drug Design & Development Review, VIII (IV), 1-14

TURABIAN

    TURABIAN : Rehman, Bushra, Sadia Aslam, and Safia Ahmed. "Optimization of Pseudomonas putida SOL10 Biosurfactant Production Using Cost-Effective Substrates." Global Drug Design & Development Review VIII, no. IV (2023): 1-14. https://doi.org/10.31703/gdddr.2023(VIII-IV).01