ARTICLE

INVESTIGATION OF PLACENTAL MODIFICATIONS IN PATIENTS WITH PREECLAMPSIA EXAMINATION USING LIGHT AND ELECTRON MICROSCOPY

04 Pages : 29-33

http://dx.doi.org/10.31703/gdddr.2023(VIII-II).04      10.31703/gdddr.2023(VIII-II).04      Published : Jun 2023

Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy

    Preeclampsia can have an effect on both the mother's health as well as the unborn child, and it only occurs during gestation and after delivery. Proteins in urine as well as high levels of blood pressure are two signs of this disease that advances swiftly. The study's goal was to look at the histology and to examine for ultrastructural alterations in the placentas of preeclampsia-affected pregnancies. Slices of paraffin were made from placenta specimens used for light microscopic analysis while an electron microscope with scanning technology was employed to produce and observe specimens for ultrastructural analysis. The syncytiotrophoblast layer nuclei were found to be arranged in many sprouts and lengthy anastomosing strands, according to light microscopic examinations. The foetal placental capillaries receded up to total absence as the villus connective tissue core gradually contracted. Endothelial degeneration and atheromatous changes were present in placental stem arteries, whereas endothelial degeneration, escalating fibrosis, and obliteration were seen in lower decidual arterioles. These results are compatible with a rise in fetoplacental vascular impedance, which was shown to be present when lacking end-diastolic flow velocity was established that it existed in the umbilical artery just before birth. The results clarify how this issue has interfered with the movement of nutrients and gas.

    Placental Modification, Preeclampsia, Microscopy, Ultrastructural Alteration
    (1) Nighat Ara
    Associate Professor, Department of Anatomy, Nowshera Medical College, Nowshera, KP, Pakistan.
    (2) Waqas Ali
    Senior Lecturer, Department of Anatomy, Nowshera Medical College, Nowshera, KP, Pakistan.
    (3) Muhammad Kabir Khan Afridi
    Lecturer, Department of Anatomy, Khyber Medical University Institute of Medical Sciences, Kohat, KP, Pakistan.
    (4) Mehak e Fatima
    Lecturer, Department of Forensic Medicine, Rawal Institute of Health and Sciences, Islamabad, Pakistan.
    (5) Noor Ul Haram
    Bachelor of Surgery and Bachelor of Medicine, Jinnah Medical College, Peshawar, KP, Pakistan.
  • Aplin, J. D., Myers, J., Timms, K., & Westwood, M. (2020). Tracking placental development in health and disease. Nature Reviews Endocrinology, 16(9), 479–494. https://doi.org/10.1038/s41574-020-0372-6
  • Baschat, A., & Hecher, K. (2004). Fetal growth restriction due to placental disease. Seminars in Perinatology, 28(1), 67–80. https://doi.org/10.1053/j.semperi.2003.10.014
  • Errera, M., Kohly, R. P., & Da Cruz, L. (2013). Pregnancy-associated Retinal Diseases and Their Management. Survey of Ophthalmology, 58(2), 127–142. https://doi.org/10.1016/j.survophthal.2012.08.001
  • Han, J. Y., Kim, Y., Cho, G. J., Roh, G. S., Kim, H. J., Choi, W. S., Paik, W. Y., Rho, G., Kang, S. O., & Choi, W. (2006). Altered gene expression of caspase-10, death receptor-3 and IGFBP-3 in preeclamptic placentas. PubMed, 22(2), 168–174. https://pubmed.ncbi.nlm.nih.gov/17085968
  • Huppertz, B. (2011). Placental pathology in pregnancy complications. Thrombosis Research, 127, S96–S99. https://doi.org/10.1016/s0049-3848(11)70026-3
  • Huppertz, B. (2010). IFPA Award in Placentology Lecture: Biology of the placental syncytiotrophoblast – Myths and facts. Placenta, 31, S75–S81. https://doi.org/10.1016/j.placenta.2009.12.001
  • Khong, T. Y. (2004). Placental vascular development and neonatal outcome. Seminars in Neonatology, 9(4), 255–263. https://doi.org/10.1016/j.siny.2003.11.010
  • Krebs, C., Macara, L. M., Leiser, R., Bowman, A., Greer, I. A., & Kingdom, J. (1996). Intrauterine growth restriction with absent end-diastolic flow velocity in the umbilical artery is associated with maldevelopment of the placental terminal villous tree. American Journal of Obstetrics and Gynecology, 175(6), 1534–1542. https://doi.org/10.1016/s0002-9378(96)70103-5
  • Landon, M. B., Galan, H. L., Jauniaux, E. R., Driscoll, D. A., Berghella, V., Grobman, W. A., ... & Cahill, A. G. (2020). Obstetrics: Normal and Problem Pregnancies E-Book. Elsevier Health Sciences.
  • Livingston, J. A., Chin, R., Haddad, B., McKinney, E. C., Ahokas, R. A., & Sibai, B. M. (2000). Reductions of vascular endothelial growth factor and placental growth factor concentrations in severe preeclampsia. American Journal of Obstetrics and Gynecology, 183(6), 1554–1557. https://doi.org/10.1067/mob.2000.108022
  • Red-Horse, K., Zhou, Y., Genbacev, O., Prakobphol, A., Foulk, R. A., McMaster, M. T., & Fisher, S. J. (2004). Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface. Journal of Clinical Investigation, 114(6), 744– 754. https://doi.org/10.1172/jci200422991
  • Smith, T. C., Kirkpatrick, D. L., Kovilam, O., & Gold, R. (2015). Immunomodulatory role of vitamin D in the pathogenesis of preeclampsia. Expert Review of Clinical Immunology, 11(9), 1055–1063. https://doi.org/10.1586/1744666x.2015.1056780
  • Weissgerber, T. L., & Wolfe, L. A. (2006). Physiological adaptation in early human pregnancy: adaptation to balance maternal- fetal demands. Applied Physiology, Nutrition, and Metabolism, 31(1), 1–11. https://doi.org/10.1139/h05-003
  • Zhou, Y., Damsky, C. H., Chiu, K., Roberts, J. M., & Fisher, S. M. (1993). Preeclampsia is associated with abnormal expression of adhesion molecules by invasive cytotrophoblasts. Journal of Clinical Investigation, 91(3), 950–960. https://doi.org/10.1172/jci116316

Cite this article

    APA : Ara, N., Ali, W., & Afridi, M. K. K. (2023). Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy. Global Drug Design & Development Review, VIII(II), 29-33. https://doi.org/10.31703/gdddr.2023(VIII-II).04
    CHICAGO : Ara, Nighat, Waqas Ali, and Muhammad Kabir Khan Afridi. 2023. "Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy." Global Drug Design & Development Review, VIII (II): 29-33 doi: 10.31703/gdddr.2023(VIII-II).04
    HARVARD : ARA, N., ALI, W. & AFRIDI, M. K. K. 2023. Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy. Global Drug Design & Development Review, VIII, 29-33.
    MHRA : Ara, Nighat, Waqas Ali, and Muhammad Kabir Khan Afridi. 2023. "Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy." Global Drug Design & Development Review, VIII: 29-33
    MLA : Ara, Nighat, Waqas Ali, and Muhammad Kabir Khan Afridi. "Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy." Global Drug Design & Development Review, VIII.II (2023): 29-33 Print.
    OXFORD : Ara, Nighat, Ali, Waqas, and Afridi, Muhammad Kabir Khan (2023), "Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy", Global Drug Design & Development Review, VIII (II), 29-33
    TURABIAN : Ara, Nighat, Waqas Ali, and Muhammad Kabir Khan Afridi. "Investigation of Placental Modifications in Patients with Preeclampsia: Examination using Light and Electron Microscopy." Global Drug Design & Development Review VIII, no. II (2023): 29-33. https://doi.org/10.31703/gdddr.2023(VIII-II).04