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EVALUATION OF THE ANTIINFLAMMATORY PHARMACOLOGICAL EFFECTS OF ARTEMISIA ANNUA EXTRACTS ARTEMISININ IN VITRO AND IN VIVO

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02 Pages : 7-14

http://dx.doi.org/10.31703/gdddr.2023(VIII-II).02      10.31703/gdddr.2023(VIII-II).02      Published : Jun 2023

Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo

Abstract

    Artemisia annua L. is acknowledged as a traditional medicinal plant bearing potent antimalarial, antileishmanial, antibacterial, antifungal and antioxidant potential. Therefore, this study was carried out to explore the anti-inflammatory impact of A. annua both in vitro and in vivo in rabbit models. We procured fresh A. annua plant material from local nurseries and Artemisinin was extracted from its leaves. The anti-inflammatory potential of the herb was found significant (p<0.05) in terms of IC-50 against the control. For in vivo trial, 50 rabbits were procured and 5-HT were injected parenterally into their paws to generate inflammation and were subsequently treated with Artemisinin, acetylsalicylic acid, and normal saline for the control group. Rabbits were treated using three different concentrations of Artemisinin @ 100mg/ml, 200mg/ml and 500mg/ml in Group A, B and C, respectively, while, Group D using Acetylsalicylic acid @ 80mg/Kg and Group E served as control. The rabbits of Group C demonstrated the greatest reduction in inflammation in the inflamed paws of model rabbits within the first four hours of treatment and significantly (p<0.05) reduced the swelling after two and four hours by 40 and 60 per cent, respectively. Artemisinin has a thus potent anti-inflammatory effect and can be employed as commercial anti inflammatory medicine.

    Anti-inflammatory, Cytokines, Inflammatory mediators, Interleukines, Potent drugs

    (1) Muhammad Saqib
    Institute of Physiology and Pharmacology, University of Agriculture Faisalabad, Punjab, Pakistan.
    (2) Samiyah Tasleem
    Department of Microbiology, FUUAST and University of Karachi, Sindh, Pakistan.
    (3) Kamran Afzal
    Senior Instructor, Department of Biological and Biomedical Sciences Aga Khan University, Pakistan.
    (4) Faisal Rashid
    Faculty of Pharmacy, Gomal University Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
    (5) Asma Siddiq
    Institute of Physiology and Pharmacology, University of Agriculture Faisalabad, Punjab, Pakistan.
    (6) Ali Asghar Bhutto
    Liaquat University of Medical and Health Sciences Jamshoro, Sindh, Pakistan.
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References

  • Allemailem, K. S. (2022). Aqueous Extract of Artemisia annua Shows In Vitro Antimicrobial Activity and an In Vivo Chemopreventive Effect in a Small-Cell Lung Cancer Model. Plants, 11(23), 3341. https://doi.org/10.3390/plants11233341
  • Bedouhène, S., Moulti-Mati, F., Hurtado- Nedelec, M., Dang, P. M.-C., & El-Benna, J. (2017). Luminol-amplified chemiluminescence detects mainly superoxide anion produced by human neutrophils. American Journal of blood research, 7(4), 41. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545213
  • Bode, J. G., Ehlting, C., & Häussinger, D. (2012). The macrophage response towards LPS and its control through the p38MAPK–STAT3 axis. Cellular Signalling, 24(6), 1185–1194. https://doi.org/10.1016/j.cellsig.2012.01.018
  • Chu, Y., Wang, H., Chen, J. M., & Hou, Y. (2014). New Sesquiterpene and Polymethoxy- Flavonoids from Artemisia annua L. Pharmacognosy Magazine, 10(39), 213. 9 https://doi.org/10.4103/0973-1296.13735
  • Efferth, T., & Oesch, F. (2021). The immunosuppressive activity of artemisinin- type drugs towards inflammatory and autoimmune diseases. Medicinal Research Reviews, 41(6), 3023–3061. https://doi.org/10.1002/med.21842
  • Ekiert, H., ÅšwiÄ…tkowska, J., Klin, P., & Rzepiela, A. (2021). Artemisia annua – Importance in Traditional Medicine and the Current State of Knowledge on the Chemistry, Biological Activity and Possible Applications. Planta Medica, 87(08), 584–599. https://doi.org/10.1055/a-1345-9528
  • Habib, M., & Waheed, I. (2013). Evaluation of anti-nociceptive, anti-inflammatory and antipyretic activities of Artemisia scoparia hydromethanolic extract. Journal of Ethnopharmacology, 145(1), 18–24. https://doi.org/10.1016/j.jep.2012.10.022
  • Helfand, S. L., Werkmeister, J. A., & Roder, J. C. (1982). Chemiluminescence response of human natural killer cells. I. The relationship between target cell binding, chemiluminescence, and cytolysis. Journal of Experimental Medicine, 156(2), 492–505. https://doi.org/10.1084/jem.156.2.492
  • Jeong, S., Kim, S., & Min, H. (2018). In vitro anti- inflammatory activity of the Artemisia montana leaf ethanol extract in macrophage RAW 264.7 cells. Food and Agricultural Immunology, 29(1), 688–698. https://doi.org/10.1080/09540105.2018.1439454
  • Kim, W., Choi, W. Y., Lee, S., Kim, W. H., Lee, D. H., Sohn, U. D., Shin, H. S., & Kim, W. (2014). Anti-inflammatory, Antioxidant and Antimicrobial Effects of Artemisinin Extracts from Artemisia annual. The Korean Journal of Physiology and Pharmacology, 19(1), 21. https://doi.org/10.4196/kjpp.2015.19.1.21
  • Organization, W. H. (2006). WHO monograph on good agricultural and collection practices (GACP) for Artemisia annua L: World Health Organization
  • Parameswari, P., Devika, R., & Vijayaraghavan, P. (2019). In vitro anti-inflammatory and antimicrobial potential of a leaf extract from Artemisia nilagirica (Clarke) Pamp. Saudi Journal of Biological Sciences, 26(3), 460– 463. https://doi.org/10.1016/j.sjbs.2018.09.005
  • Patil, K. R., Patil, C. R., Unger, B. S., Goyal, S. N., Belemkar, S., Surana, S. J., Ojha, S., & Patil, C. R. (2019). Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. International Journal of Molecular Sciences, 20(18), 4367. https://doi.org/10.3390/ijms20184367
  • Pellicer, J., Saslis-Lagoudakis, C. H., Carrió, E., Ernst, M., Garnatje, T., Grace, O. M., Gras, A., Mumbrú, M., Vallès, J., Vitales, D., & Rønsted, N. (2018). A phylogenetic road map to antimalarial Artemisia species. Journal of Ethnopharmacology, 225, 1–9. https://doi.org/10.1016/j.jep.2018.06.030
  • Salah-Abbès, J. B., Abbès, S., Ouanes, Z., Houas, Z., Abdel-Wahhab, M. A., Bacha, H., & Oueslati, R. (2008). Tunisian radish extract (Raphanus sativus) enhances the antioxidant status and protects against oxidative stress induced by zearalenone in Balb/c mice. Journal of Applied Toxicology, 28(1), 6–14. https://doi.org/10.1002/jat.1240
  • Shi, C., Li, H., Yang, Y., & Hou, L. (2015). Anti- Inflammatory and Immunoregulatory Functions of Artemisinin and Its Derivatives. Mediators of Inflammation, 2015, 1–7. https://doi.org/10.1155/2015/435713
  • Su, X., & Miller, L. H. (2015). The discovery of artemisinin and the Nobel Prize in Physiology or Medicine. Science China-life Sciences, 58(11), 1175–1179. https://doi.org/10.1007/s11427-015-4948-7
  • Willcox, M. (2009). ArtemisiaSpecies: From Traditional Medicines to Modern Antimalarials—and Back Again. Journal of Alternative and Complementary Medicine, 15(2), 101–109. https://doi.org/10.1089/acm.2008.0327
  • Yun, C., Jung, Y., Chun, W., Yang, B., Ryu, J., Lim, C., Kim, J. H., Kim, H., & Cho, S. (2016). Anti-Inflammatory Effects of ArtemisiaLeaf Extract in Mice with Contact Dermatitis In Vitroand In Vivo. Mediators of Inflammation, 2016, 1–8. https://doi.org/10.1155/2016/8027537

Cite this article

APA

    APA : Saqib, M., Tasleem, S., & Afzal, K. (2023). Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo. Global Drug Design & Development Review, VIII(II), 7-14. https://doi.org/10.31703/gdddr.2023(VIII-II).02

CHICAGO

    CHICAGO : Saqib, Muhammad, Samiyah Tasleem, and Kamran Afzal. 2023. "Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo." Global Drug Design & Development Review, VIII (II): 7-14 doi: 10.31703/gdddr.2023(VIII-II).02

HARVARD

    HARVARD : SAQIB, M., TASLEEM, S. & AFZAL, K. 2023. Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo. Global Drug Design & Development Review, VIII, 7-14.

MHRA

    MHRA : Saqib, Muhammad, Samiyah Tasleem, and Kamran Afzal. 2023. "Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo." Global Drug Design & Development Review, VIII: 7-14

MLA

    MLA : Saqib, Muhammad, Samiyah Tasleem, and Kamran Afzal. "Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo." Global Drug Design & Development Review, VIII.II (2023): 7-14 Print.

OXFORD

    OXFORD : Saqib, Muhammad, Tasleem, Samiyah, and Afzal, Kamran (2023), "Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo", Global Drug Design & Development Review, VIII (II), 7-14

TURABIAN

    TURABIAN : Saqib, Muhammad, Samiyah Tasleem, and Kamran Afzal. "Evaluation Of The Anti-Inflammatory Pharmacological Effects Of Artemisia Annua Extracts Artemisinin In Vitro And In Vivo." Global Drug Design & Development Review VIII, no. II (2023): 7-14. https://doi.org/10.31703/gdddr.2023(VIII-II).02